As 2025 unfolds, a brand new wave of Czech startups is able to make its mark, bringing contemporary concepts, progressive applied sciences, and an unstoppable entrepreneurial drive. These rising firms usually are not simply getting into the scene; they’re set to disrupt industries, additional establishing the Czech Republic as a number one vacation spot for startups within the area.
In recent times, the nation has been drawing the eye of each buyers and entrepreneurs. Cities like Prague, Brno, and Ostrava wish to grow to be key centres of innovation in Central and Jap Europe, notably in fields like ICT, software program improvement, and cell apps. To nurture this progress, the Czech Startup Affiliation was created, aiming to make sure a supportive setting that helps startups thrive and retains the nation on the slicing fringe of the worldwide market.
On this article, we delve into the Czech entrepreneurial scene to focus on 10 promising startups, all based after 2022, which are more likely to make a notable influence throughout completely different sectors.
Adalid Sciences: Based in 2024 and based mostly in Prague, this biotechnology startup is devoted to advancing pharmaceutical options by cutting-edge lipid nanoparticle (LNP) know-how. By leveraging LNPs, the corporate goals to reinforce drug stability and bioavailability, bettering therapeutic efficacy whereas minimising unintended effects.
With intensive experience in artificial chemistry, biochemistry, and nanoparticle formulation, Adalid Sciences seamlessly integrates its options into shopper workflows. They’ve secured over €8 million in funding to drive innovation in nucleic acid supply programs and associated fields.
CRUXO: This data-driven startup has developed an progressive platform designed to assist retailers enhance their income by automating promoting and offering real-time insights. It simplifies managing focused advertisements, boosts shopper engagement, and will increase earnings from on-site media in sectors like magnificence, electronics, and groceries. Based in 2022 in Prague, the startup has raised €800k to leverage experience in retail, vendor advertising and marketing, and on-line media to ship progressive options for companies.
Flowlance: Launched in 2022, Flowlance is a platform designed to assist creators flip their data into earnings effortlessly. It permits customers to promote companies, digital merchandise, and memberships, handle bookings, acquire funds, and construct an expert on-line presence, all from one platform. With easy-to-use instruments, creators can launch an internet site, monitor gross sales, and optimise their enterprise progress with no need technical expertise. Based mostly in Prague they’ve raised 500k in funding.
Kardi AI: Based mostly in Olomouc, Kardi AI focuses on offering worry-free coronary heart care from dwelling. Kardi AI combines commercially out there trackers with cutting-edge AI to watch coronary heart well being, making it simpler for people to trace and handle their cardiovascular well-being.
By means of AI, machine studying, and healthcare, the pioneering startup goals to rework the way in which coronary heart well being is monitored exterior of medical services. Based in 2022 and with a funding of €1.9 million, Kardi AI is positioning itself as a frontrunner in distant coronary heart care, guaranteeing early detection and steady monitoring for customers.
Lakmoos: This market analysis startup makes use of neuro-symbolic AI to simulate client behaviour. Lakmoos platform supplies prompt, correct market insights, enabling companies to collect real-time knowledge with out the necessity for human respondents or lengthy, expensive analysis processes. By leveraging AI, they provide a cheap, quick, and safe resolution for industries like automotive, banking, and telecommunications.
Headquartered in Brno, Lakmoos goals to disrupt conventional market analysis with an automatic, data-driven strategy to client insights. Launched in 2023, the startup landed €510k in funding to gas its mission of remodeling how companies collect client knowledge.
Langtail: Prague-based, Langtail is a platform that simplifies the method of testing AI purposes, particularly those who use giant language fashions. Based in 2023, their progressive platform helps groups guarantee their AI works properly by testing its responses and bettering accuracy, all by a easy interface. This makes enhancing AI safety and efficiency simpler for customers, even these with out intensive technical understanding. Langtail has efficiently raised €954k.
Merchantee: Launched in 2023, Merchantee is an e-commerce SaaS platform designed to assist on-line sellers handle their gross sales throughout EU marketplaces. Based mostly in Prague, Merchantee simplifies the complexities of multi-marketplace promoting, permitting companies to seamlessly monitor, handle, and enhance their gross sales in a single unified platform. With a funding of €614k, the progressive startup is empowering e-commerce entrepreneurs to increase their attain and improve operational effectivity throughout the European Union’s various on-line markets.
Rezolv Vitality: Based mostly in Prague, this unbiased renewable vitality producer focuses on wind, photo voltaic, and battery storage options. The startup supplies subsidy-free renewable vitality at steady costs for industrial and business customers, guaranteeing entry to dependable sustainable energy. Based in 2022, Rezolv Vitality has raised €90 million, marking important investor confidence in its mission to construct a sustainable energy portfolio throughout Central and South Jap Europe.
RobosizeME: Based in 2022, this automation-focused startup is revolutionising operational processes within the hospitality trade by streamlining repetitive duties. With its automation as a service (AaaS) mannequin, the startup handles every little thing from figuring out automation alternatives to constructing, implementing, and sustaining options. By automating duties equivalent to fee loading, fee monitoring, and cost reconciliation, the startup allows lodge workers to give attention to delivering higher visitor experiences. The Olomouc-based startup has raised €1 million to scale its progressive automation companies.
Salted CX: Based mostly in Prague, Salted CX assists firms in offering higher customer support. By utilizing AI, they centralise knowledge from all buyer interactions both, chats, emails or telephone calls, to analyse every dialogue, establish developments, and level out areas for enchancment.
The platform makes it easy to trace efficiency, search for particular points, and generate reviews to establish points rapidly. For example, it may spotlight areas the place purchasers are irritated or the place brokers require extra help. On this method, firms could preserve buyer satisfaction, improve service, and resolve issues extra rapidly. Based in 2022, Salted CX has raised €1.4 million to develop its software program options.
By the way in which: If you happen to’re a company or investor in search of thrilling startups in a particular marketplace for a possible funding or acquisition, take a look at our Startup Sourcing Service!3
